Seminars and Events

As part of their training grant committment, each trainee gives two presentations during their grant cycle: a journal club and a presentation of their research. These seminars take place on Tuesdays at 4 p.m. and are will be held virtually until further notice. The full schedule for the 2020-21 academic year is below.

Every year, trainees select a theme for the coming year. This year the theme is:

Global Implications of Microbial Interactions

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CANCELLED September 8, 2020 - MHD Journal Club, Troy King

“Adaptive evolution of virulence and persistence in carbapenem-resistant Klebsiella pneumoniae”

Christoph M. Ernst et. al.

https://www.nature.com/articles/s41591-020-0825-4

 

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September 22, 2020 - MHD Journal Club, Mary Swaney

“Respiratory Bacteria Stabilize and Promote Airborne Transmission of Influenza A Virus”

Hannah M. Rowe et. al

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October 6, 2020 - MHD Journal Club, Margaret Thairu

“Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens”

Pilar Vesga et. al.

Margaret Thairu

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October 20, 2020 - MHD Journal Club, Morgan Giese

“Growth rate alterations of human colorectal cancer cells by 157 gut bacteria”

Rahwa Taddese et. al.

https://pubmed.ncbi.nlm.nih.gov/32915102/

Morgan Giese

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November 3, 2020 - MHD Journal Club, Dianiris Luciano-Rosario

“Conidial Melanin of the Human-Pathogenic Fungus Aspergillus fumigatusDisrupts Cell Autonomous Defenses in Amoebae”

Iuliia Ferling et. al.

https://mbio.asm.org/content/11/3/e00862-20

Host: Dianiris Luciano-Rosario

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November 17, 2020 - MHD Seminar, Mark Horton

“Evasion of innate immune response by emergent pathogen Candida auris”

Mark Horton
Graduate Student, Nett Lab

Abstract

Candida auris is an emergent fungal pathogen that causes invasive disease with a mortality rate approaching 60%. Neutrophils are innate immune cells critical for controlling many fungal infections. However, C. auris is capable of evading recognition and killing by neutrophils. C. auris has a cell wall composed of inner immunostimulatory polysaccharides such as β-glucans, which are covered by an outer layer of branched mannans on glycoproteins. We hypothesize the mannan polysaccharide layer in C. auris is poorly recognized by neutrophils and functions to mask inner β-glucans. To disrupt mannans, we generated mutants in genes which encode proteins that function early in the mannosylation pathways. Using ex vivo assays with human neutrophils, we demonstrated pmr1Δ and van1Δ mannan mutants to be 3.5-fold and 2.5-fold more susceptible to neutrophil phagocytosis, respectively, compared to the parent strain. The mutants were also more susceptible to neutrophil killing. Complementation of VAN1 reversed these phenotypes. In the zebrafish hindbrain infection model, the mannan mutants displayed decreased fungal burden compared to the parent strain. Transmission electron microscopy and 1H NMR showed a large reduction in mannan content in the mutants, and immunofluorescence microscopy showed a corresponding increase in exposed β-glucans compared to the parent strain. These studies suggest C. auris cell wall mannans participate in neutrophil evasion by masking β-glucans.

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December 1, 2020 - MHD Seminar, Troy King

MHD Seminar

Troy King

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December 15, 2020 - MHD Journal Club, Lindsay Taylor

MHD Journal Club

Lindsay Taylor

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February 2, 2021 - MHD Journal Club, Bryan Vonasek

MHD Journal Club

Bryan Vonasek

 

February 16, 2021 - MHD Seminar, Lindsay Taylor

MHD Seminar

Lindsay Taylor

March 2, 2021 - MHD Seminar, Mary Swaney

MHD Seminar

Mary Swaney

March 16, 2021 - MHD Seminar, Morgan Giese

MHD Seminar

Morgan Giese

April 6, 2021 - MHD Journal Club, Mark Horton

MHD Journal Club

Mark Horton

April 20, 2021 - MHD Seminar, Dianiris Luciano-Rosario

MHD Seminar

Dianiris Luciano-Rosario

May 4, 2021 - MHD Seminar, Margaret Thairu

MHD Seminar

Margaret Thairu

May 18, 2021 - MHD Seminar, Bryan Vonasek

MHD Seminar

Bryan Vonasek